It’s been pouring with rain outside since first thing this morning, so a good time to dust off the microscope and do a bit of photomicroscopy.
Here is a 10-frame mosaic of a Thyroid Gland taken with the Canon 5D MkII.
I used the research trinocular microscope and the Canon 5D MkII to grab a x20 focus-stacked image of the hornet’s eye which you can see HERE.
Got today’s EPOD with my focus-stacked photomicromosaic of the Diatom Exhibition slide. Thank you Jim for continuing to publish my work 🙂
This image is a 42-frame micromosaic taken with the Canon 5D MkII and a research trinocular microscope at magnification x50. It is the cross-section of a Curcurbits stem, an image I have done before, but not at this magnification. The resulting 42-frame mosaic came out at 25,000 x 23,000 pixels and is the largest photomicromosaic I have assembled to date. Well I guess Photoshop CS3 did the assembling using the Photomerge function, which also does a superb job on the blending as well.
Be warned – it took over an hour for Photoshop to put this together for me and I run a Quadcore 2.5GHz Intel machine with 8 Gig of RAM and Windows 7 64-bit. So it is not a lightweight system and yet it took this long to assemble. Just flattening the final image took nearly half an hour!!
These massive mosaics are great fun (I wish I had enough clear skies to put together massive deep-sky mosaics – but even the mini-WASP array won’t help me out too much with that problem) – but in future I will try to stick to mosaics of about half this size, so around 20-frames.
This is a photomicroscopy image of the cross-section of a Lily ovary. But this is a microscope image with a difference! This is a 31-frame mosaic taken with the Canon 5D MkII – so the original image is a massive 20,000 x 20,000 pixels in size – that’s a 0.4 Gigapixel image in real money. My computer struggled with even the simplest Photoshop action with that size of image so I won’t go beyond 6 x 6 frames for any 5D MkII mosaic in future.