This image is a 60-frame Helicon Focus-stacked image of the eye region of a Hornet.  Camera Canon 5D MkII and research trinocular microscope.

A 69-frame focus-stack image of a Hornet’s leg using Helicon Focus for the stacking, a Canon 5D MkII for the imaging and a research trinocular microscope for the x20 magnification.

Put together using Helicon Focus, this is a 50-frame focus stacked image of the eye region of a Hornet taken using a Canon 5D MkII on a research trinocular microscope at magnification x20.

Instead of sitting the Butterfly on a microscope slide which gave a blurred flare in bright regions – this image was taken with the Butterfly on a black velvet background – no flare 🙂

For this image I increased the focus depth-resolution and also slightly changed the Helicon Focus stacking parameters for a little more detail.

As it has been drizzling all day I have been doing some microscopy while my wife worked in the greenhouse.  Knowing I was working with the microscope she brought me in a dead butterfly from the greenhouse.  It went straight under the microscope for a 22-frame focus-stacked image stitched together using that brilliant piece of software Helicon Focus.

A very quick mosaic and process to check the system still runs o.k. and it was a rainy morning with nothing better to do 🙂